Purification and Characterization of the Crown Gall-specific Enzyme, Octopine Synthase
نویسندگان
چکیده
منابع مشابه
Unusual plasmid DNA organization in an octopine crown gall tumor.
A cloned tobacco crown gall tumor, 1595501, incited by A. tumefaciens strain 15955 was studied. Molecular analysis of the organization of the T-DNA by means of Southern transfer and hybridization techniques indicated that the 1595501 tumor has about 10 copies of TL DNA, five of which are complete TL DNA, whereas most octopine tumors have only one to two copies of complete TL DNA. Hybridization ...
متن کاملSizes and map positions of several plasmid-DNA-encoded transcripts in octopine-type crown gall tumors.
The sizes and relative map positions of RNA molecules encoded by the plasmid DNA of several octopine-type tobacco crown gall tumors were determined by blot hybridization. At least six discrete polyadenylylated RNAs (sizes 0.73-1.75 kilobases) were detected in octopine-producing tumors (lines A6S/2, E9, and 15955/01). In one tumor (line 15955/1) in which octopine could not be detected, one of th...
متن کاملPromotion of crown-gall tumor growth by lysopine, octopine, nopaline, and carnosine.
The growth of crown-gall tumors on primary bean leaves (Phaseolus vulgaris L. cv. "Pinto") was promoted by the addition of d-lysopine, d-octopine, l-carnosine, or nopaline. Assayed on tumors induced by Agrobacterium tumefaciens strain B6, the relative activity was octopine = carnosine > lysopine >> nopaline; assayed on tumors induced by A. tumefaciens strain T-37, which induces tumors which for...
متن کاملInduction of responsiveness to octopine and lysopine in crown-gall tumors.
Bean leaf tumors induced by Agrobacterium tumefaciens strain 181 show enhanced growth in response to octopine or lysopine only if one of these compounds is present during the period of tumor induction. Either compound applied during this period results in tumors which subsequently respond to both. The combined action of the bacterium plus octopine or lysopine at induction is proposed to induce ...
متن کاملAgrobacterium tumefaciens mutants affected in crown gall tumorigenesis and octopine catabolism.
Mutants of Agrobacterium tumefaciens which affect virulence or the ability to catabolize octopine were isolated after Tn5-induced mutagenesis. Of 8,900 colonies tested, 7 mutants with Tn5 insertions in a specific region of other Ti plasmid unable to catabolize octopine were isolated. Thirty-seven mutants affected in tumorigenesis resulted from insertions in the Ti plasmid and the Agrobacterium ...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1980
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.65.5.949